DNA

Part:BBa_K2350008:Design

Designed by: CHEN-CHIA JIANG   Group: iGEM17_NYMU-Taipei   (2017-10-25)


Lycopene cyclase downstream in PCC7942


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 442
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 435


Design Notes

PCR primers for S. elongatus PCC7942 genomic DNA
Forward: ATCGTGCTTTGCAGCGGGTG
Reverse: CGGCGTTACCGTCACGTAGT

Source

Synechoccocus elongatus PCC7942 genomic DNA.

References

Cunningham, F. X., Sun, Z., Chamovitz, D., Hirschberg, J., & Gantt, E. (1994). Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. The Plant Cell, 6(8), 1107–1121.